clone method meaning in English
克隆方式；复制方法
Examples
- To acquire the polyclonal antibody of hbaf53 , we made use of several molecular cloning methods
为了获得hbaf53的多克隆抗体，本实验采用基因工程的方法制备抗原。 - The traditional homogene cloning method was modified on two aspects : first , homo - primers was designed at the sites with minimum degeneracy instead of maximum homology , secondly , some " n " bases were replaced with inosine in degenerate codes , resulting hi the degeneracy reduced from over 1000 times to below 100 times
改进后的同源引物简并度从1000倍以上降低至100倍以下，利用rt一pcr成功地扩增出一条630bp的盐藻cdna片段，该片段与其它物种的烯醇酶基因具有很高的相似性。 - The complete nucleotide sequences of two strain viruses were determined . ten pairs of primers were designed based on the nucleotide sequences of tbev sofjin - ho , oshima5 - 10 by dnastar software . the virus cdna was expanded by rt - pcr in ten parts . sequenced by clones method . 5 " terminal and 3 " terminal cdna was expanded by race method . the nucleotide sequences and deduced amino acid sequences of two strain viruses were compared with the sequences in genbank by dnastar software
根据genbank中已公布的tbevsofjin - ho株和oshima5 - 10株核苷酸序列利用dnastar软件设计10对引物，利用rt - pcr技术分段扩增病毒cdna ，克隆法测序。病毒基因组5末端和3末端序列采用race法扩增。测序结果用dnastar软件拼接，在genbank中检索6株tbev编码区核苷酸序列、 12株tbeve蛋白核苷酸序列，并在dnastar上进行核苷酸序列、由此推导的氨基酸序列比较及构建系统发生树。 - Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。